Protocols In this page you will find protocols and documentation that are used in our laboratory – if you have any remarks or suggestions in regard to these protocols, please don’t hesitate to contact at – zarivach.lab[at]gmail.com. Thanks! Molecular biology Introduction to CloningTransformation of competent cells with plasmid DNA – Heat shock methodTransformation of competent cells with plasmid DNA – Electroporation methodPreparation of electrocompetent cellsPreparation of colloidal stain Protein expression Novagen pET system manual Protein purification French press (Thermo Scientific)FPLC SOPFPLC AKTA prime plus SOPGuide to PipettingSX20 Stopped Flow Spectrometer“TUTTNAUER” Old Autoclave Operating ProcedureOperate procedure for Emulsiflex high presure homogenizer Crystallography documentation CCP4 User ManualCoot User ManualCoot tutorial for low-resolution casesImosflm TutorialPhenix Manual General methods Magnetite synthesis protocolGold cluster synthesisITC (low-volume Nano ITC calorimeter, TA Instruments) for the study of CDF proteins’ CTD binding to metalsTrp-fluorescence spectrometry (Fluorolog-3, HORIBA Scientific) for the study of CDF proteins’ CTD binding to metalsEating in flights – Raz Zarivach’s protocol for free upgraded meals Lab Mates Lab FAQ from RocheThe Source Book from CambrexBench Guide from QIAGEN Biochemistry Peptide mass fingerprintingPreparation of Tricine-SDS-PAGEPreparation of 15% SDS-Polyacrylamide gelWestern BlottingRemoval of Thrombin associated affinity tags (suspension)Steps in large-scale recombinant protein expression and purificationNickel column preparation and maintenanceAmylose column preparation and usageProtein labelling with FITC