Papers in refereed journals
2021
Baron, N., Tupperwar, N., Orr, I., Hadad, U., Davidov, G. Zarivach, R. and Shapira, M. 2021. Distinct features of the Leishmania cap-binding protein LeishIF4E2 revealed by CRISPR-Cas9 mediated hemizygous deletion. PLOS Negl Dis 2021 Mar 24;15(3):e0008352. doi: 10.1371/journal.pntd.0008352. eCollection 2021 Mar. PMID: 33760809
Shrivastava R, Tupperwar N, Schwartz B, Baron N, Shapira M. (2021) LeishIF4E-5 is a promastigotes-specific cap-binding protein in Leishmania. Int J Mol Sci. 2021 Apr 12;22(8):3979. doi: 10.3390/ijms22083979.PMID: 33921489
Richa Amiya and Michal Shapira. (2021) ZnJ6 Is a Thylakoid Membrane DnaJ-Like Chaperone with Oxidizing Activity in Chlamydomonas reinhardtii. Int J Mol Sci Jan 24;22(3):1136. doi: 10.3390/ijms22031136.
2019–2020
Tupperwar N, Meleppattu S, Shrivastava R, Baron N, Gilad A, Wagner G, Léger-Abraham M, Shapira M. (2020). A newly identified Leishmania IF4E-interacting protein, Leish4E-IP2, modulates that activity of cap-binding protein paralogs. Nucleic Acids Res. 2020 May 7;48(8):4405-4417. doi: 10.1093/nar/gkaa173
Shrivastava, R., Drory-Retwitzer, M. and Shapira, M. (2019). Nutritional stress targets LeishIF4E-3 to storage granules that contain RNA and ribosome components in Leishmania. PLOS Neg Trop Dis. Mar 14;13(3):e0007237. doi: 10.1371/journal.pntd.0007237. eCollection 2019 Mar.
Tupperwar, N., Shrivastava, R. and Shapira, M. (2019). LeishIF4E1 deletion affects the promastigotes proteome, morphology and infectivity. mSphere. 2019 Nov 13;4(6).
Rohit Shrivastava, Nitin Tupperwar, Matan-Drory-Retwitzer and Michal Shapira (2019) . Deletion of a Single LeishIF4E-3 Allele by the CRISPR-Cas9 System Alters Cell Morphology and Infectivity of Leishmania. mSphere 2019 Sep 4;4(5):e00450-19. doi: 10.1128/mSphere.00450-19
2018
Meleppattu, S., Arthanari, H., Zinoviev, A., Boeszoermenyi, A., Wagner, G., Shapira, M. and Léger-Abraham, M. (2018). Structural basis for LeishIF4E-1 modulation by an interacting protein in the human parasite Leishmania major Nucleic Acids Res. 7:3791-3801. doi: 10.1093/nar/gky194.
Doron, L., Goloubinoff, P., Shapira, M. (2018). ZnJ2 is a member of a large chaperone family in the chloroplast of photosynthetic organisms that features a DnaJ-like Zn-Finger domain. (2018) Front Mol Biosci. Feb 15;5:2. doi: 10.3389/fmolb.2018.00002. eCollection 2018.
2015–2016
Doron, L. Segal, N. and Shapira, M. Transgene expression in microalgae – from tools to applications. (2016) Front Plant Sci. 22;7:505. Invited Review.
Melappattu, S., Kamus-Elimelech D, Zinoviev, A., Orr, I and Shapira, M. (2015) The eIF3 complex of Leishmania – subunit composition and mode of recruitment to different cap-binding complexes. Nucleic Acids Res, 43:6222-35
Segal, N. and Shapira, M. (2015) Light-induced redox control of the HSP33 chaperone from Chlamydomonas reinhardtii. Plant J, 82:850-860.
2014
Doron, L., Segal, N., Gibori, H. and Shapira, M. (2014) The BSD2 ortholog in Chlamydomonas reinhardtii is a polysome-associated chaperone that co-migrates on sucrose gradient with the rbcL transcript encoding the Rubisco large subunit. Plant J, 80:345-55
Zorin, B., Grindman, O., Khozin-Goldberg I, Leu S, Shapira M, Kaye Y, Tourasse N, Vallon O, Boussiba S. (2014). Developent of a nuclear transformation system for Oleaginous green alga Lobosphaera (Parietochloris) incisa and genetic complementation of a mutant strain, deficient in arachidonic. PLoS One. 2014 Aug 18;9(8):e105223
Churkin, A., Avihoo, A., Shapira, M. and Barash, D. (2014) RNAthermsw: direct temperature simulations for predicting the location of RNA thermometers, PloS One, 9(4):e94340
2012–2013
Bocobza, S. E., Malitsky, S., Araújo, W.L., Nunes-Nesi, A., Meir, S., Shapira, M. Fernie, A.R. and Aharoni, A. (2013) Orchestration of Thiamin biosynthesis and central metabolism by combined action of the Thiamin Pyrophosphate Riboswitch and the circadian clock in Arabisopsis, Plant Cell. 25, 288-307
Zinoviev, A. and Shapira, M, (2012) Evolutionary conservation and diversification of the translation initiation apparatus in tryapnosomatids. Comparative and Functional Genomics 2012: 813718. (Invited Review) (PMC3399392)
Zinoviev, A., Manor, S. and Shapira, M. (2012) Nutritional stress affects an atypical cap-binding protein in Leishmania . RNA Biology, 9, 1450-1460
Zinoviev, A., Akum, Y., Yahav, T., and Shapira, M. (2012) Gene duplication in trypanosomatids – two stage-specific Ded1p paralogs are functionally redundant. Mol. Biochem. Parasitol. 185, 127-36.
Grundman, O, Khozin-Goldberg, I, Raveh, D, HaCohen, Z, Vyazmensky, M., Boussiba, S. and Shapira, M. (2012) Cloning, mutagenesis and characterization of the microalga Parietochloris incisa acetohydroxyacid synthase, and its possible use as an endogenous selection marker. Biotechnol Bioeng. 109, 2340-2348
2011
Zinoviev, A., Leger, M., Wagner, G and Shapira, M. (2011) A Novel 4E-Interacting Protein in Leishmania is involved in stage specific translation pathways. Nucleic Acids Research, 39(19):8404-15 (PMC3201875)
Shapira, M. and A. Zinoviev (2011) Leishmania parasites act as a Trojan horse that paralyzes the translation system of host macrophages. Cell Host Microbe 9(4): 257-9
2009–2010
David, M., Gabdank, I., Ben David, M., Zilka, A., Barash, D. and Shapira, M. (2010) Preferential translation of Hsp83 in Leishmania requires a thermosensitive polypyrimidine-rich element in the 3’ UTR and involves scanning of the 5’ UTR. RNA, 16:364-74(PMC2811665)
Yoffe, Y., Leger, M., Zinoviev, A., Zuberek, J., Darzynkiewicz, E. Wagner, G. and Shapira, M. (2009) Evolutionary adaptation of the Leishmania eIF4F complex to cap-4 binding involves changes in the eIF4E-eIF4G interactions. Nucleic Acids Res. 37:3243-53 (PMC2691829)
Raveh-Amit, H., Maissel, A., Marom, L. Elroy-Stein, O., Shapira, M. and Livneh, E. (2009) Translation control of two protein kinase Ceta by two upstream open reading frames. Mol Cell Biol 29:6140-8 (PMC2772565)
2007–2008
Cohen, A., Bocobza, S., Veksler, I., Gabdank, I., Barash, D. Aharoni, A., Shapira, M. and Kedem, K. (2008) Computational identification of three-way junctions in folded RNAs: a case study in Arabidopsis. In Silico Biol. 8:105-20
Clayton C. and Shapira M. (2007) Post-transcriptional regulation of gene expression in trypanosomes and leishmanias. (Review article) Mol. Biochem. Parasitol, 156:93-101
Lewdorowicz, M., Jemielity, J., Kierzek, R., Shapira, M., Stepinski, J. and Darzynkiewicz, E. (2007) Solid supported synthesis of 5’ –mRNA cap-4 from trypanosomatides. Nucleosides Nucleotides Nucleic Acids. 26:1329-1333
Lewdorowicz, M., Stepinski, J., Kierzek, R., Jemielity, J., Zuberek, J., Yoffe, Y., Shapira, M, Stolarski R. and Darzynkiewicz, E. (2007). Synthesis of Leishmania cap-4 intermediates, cap-2 and cap-3. Nucleosides Nucleotides Nucleic Acids. 26:1339-4
Bocobza, S., Adato, A., Mandel, T., Shapira, M., Nudler, E. and Aharoni, A. (2007). Riboswitch-dependent gene regulation and its evolution in the Plant Kingdom. Genes and Development. 21:2874-2879 (PMC2049190)
2006
Cohen, I, Sapir, Y. and Shapira, M. (2006) A conserved mechanism controls translation of Rubisco large subunit in different photosynthetic organisms. Plant Physiology, 141, 1089-1097. This paper was recommended by the “Faculty of 1000”.
Avihoo, A.§, Gabdank, I.§, Shapira, M. and Barash, D. (2006) In Silico Design of Small RNA Switches. IEEE Transactions in Nanobiosciences, 6, 4-11. (§ Equal Contribution)
Yoffe Y., Zuberek J., Lerer, A., Lewdorowicz, M., Stepinski, J., Altmann, M., Darzynkiewicz, E., and Shapira, M. (2006) eIF4E isoforms of Leishmania – binding specificities and potential roles. Eukaryotic Cell, 5, 1969-79
2005
Cohen, I., Knopf J.A., Irihimovitch, V. and Shapira, M. (2005) A proposed mechanism for the inhibitoryeffects of oxidative stress on Rubisco assembly and subunit expression. Plant Physiol. 137, 738-746.This paper was recommended by the “Faculty of 1000”
Knopf, J. and Shapira, M. (2005) Degradation of Rubisco SSU during oxidative stress triggers the aggregation of Rubisco in vivo, in Chlamydomonas reinhardtii. Planta, 222, 787-93
2004
Yosef, I. §, Irihimovitch, V. §, Dahan, I., Cohen,I., Knopf,Y., Nahum, E., Keasar, C., and Shapira, M. (2004) RNA Binding. Activity of Rubisco Large Subunit from Chlamydomonas reinhardtii. J.Biol. Chem. 279, 10148–10156 (§ – Equal contribution)
Yosef, I. Bloushtain, N., Shapira, M. and Qimron, U. (2004) Restoration of Gene Function by Homologous Recombination: From PCR to Gene Expression in One Step. Applied and Environmental Microbiology, 70, 7156-7160
Yoffe, Y., Lewdrowicz, M. Zeira, Z., Keasar, C., Orr-Dahan, I., Joanna Zuberek, J. Darzynkiewicz, E. and Shapira M. (2004) Cap binding activity of an eIF4E homologue from Leishmania. RNA, 10, 1764-1775
Lewdorowicz, M., Yoffe, Y., Zuberek, J., Jemielity, J., Stepinski, J., Kierzek,R., Stolarski, R., Shapira, M. and Edward Darzynkiewicz. (2004) Chemical synthesis and binding activity of the trypanosomatid cap-4 structure. RNA, 10, 1469-1478
2001–2002
Lapidot, M., Raveh, D., Sivan, A., Arad, S. and Shapira, M. (2002) Stable chloroplast transformation of the unicellular red algae Porphyridium sp. Plant Physiol. 129, 7-12.
Zilka, A., Garlapati, S., Dahan, E. and Yavelski V.and Shapira, M. (2001) Developmental regulation of HSP83 in Leishmania: 3’ processing and mRNA stability control transcript abundance and translation is directed by a determinant in the 3’ untranslated region. J. Biol. Chem. 276, 47922–47929
Shapira, M., Zilka, A., Garlapati, S., Dahan, E. Dahan, I. and Yavelski, V. (Nov 2001) Post Transcriptional Control of Gene Expression in Leishmania. Medical Microbiol. Immunol.190, 23-26
Oxman, T., Shapira, M., Klein, R., Avazov, N., Rabinowitz, B. (2001) Oral administration of lactobacillus induced cardioprotection. J. Alternative and Complementary Medicine 7, 345-354.
2000
Oxman, T. Shapira, M., Diver, A., Klein, R. and Rabinowitz, B. (2000) Long-Term Cardioprotection – Mechanisms of Adaptation to Ischemia. American J. Physiol.: Heart and Circulatory Physiology. 278, H1717-H1724
Irihimovitz, V. and Shapira, M. (2000) Glutathione Redox Potential Modulated by Active Oxygen Species Regulates Translation of Rubisco LSU in the Chloroplast J. Biol. Chem. 275, 16289-16295
1999
Lapidot, M., Raveh, D., Sivan, A. Arad-Malis, S. and Shapira, M. (1999) Molecular analysis of the AHAS gene from Porphyridium sp. (Rhodophyta) and from its SMM resistant mutant. J. Phycol. 35, 1233-1236.
Garlapati, S., Dahan, I., and Shapira, M. (1999) Effect of acidic pH on heat shock gene expression in Leishmania . Mol. Biochem. Parasitol. 100, 95-101
1998
Oxman, T., Arad, M., Shapiro, M., Klein, R., Elazar, E., Avezov, N. and Rabinowitz, B. (1998) Can the type of short-term ischemia determine the antiarrhythmic effect of preconditioning in the isolated rat heart? Exp. Clin. Cardiology. 3, 17-22
Garlapati, S., Aly, R. and Shapira, M. (1998) Leishmania amazonensis, Genus-specific expression from the SL RNA promoter of Leishmania amazonensis. Exp. Parasitol. 89, 266-270
1996-1997
Shapira, M. , Lers, A., Irihimovitz, V., Heifetz, P., Osmond, B., N. W. Gillham and J. E. Boynton (1997) Differential Regulation of Chloroplast Gene Expression in Chlamydomonas reinhardtii during Photoacclimation: Light Stress Transiently Suppresses Synthesis of the Rubisco LSU Protein while Enhancing Synthesis of the PS II D1 Protein. Plant Mol Biol. 33, 1001-1011
Siman-Tov, M. Aly, R. Shapira, M. and Jaffe C.L. (1996) Cloning and regulation of a stage specific protein kinase A gene from Leishmania Mol. Biochem. Parasitol. 77, 201-215
.1994–1995
Aly, R., Argaman, M. and Shapira, M. (1995) The hsp83 intergenic region in Leishmania: conservation of sequence and function across two species. Exp. Parasitology 80. 159-162.
Zilberstein D. and Shapira, M. (1994) The role of pH and temperature in the development of Leishmania parasites. Ann. Rev of Microbiol. Vol 48. 449-470
Aly, R., Argaman, M. and Shapira, M. (1994) A regulatory role for the 5′ and 3′ untranslated regions in differential expression of hsp83 in Leishmania. Nucl. Acids Res. 22, 2922-2929. Cit=64
Argaman, M., Aly,R. and Shapira,M. (1994) Expression of the hsp83 gene in the protozoan parasite Leishmania amazonensis is regulated post transcriptionally. Mol. Biochem. Parasitol. 64, 95-110
Agami, R., Aly, R. Halman, S. and Shapira, M. (1994) Functional analysis of cis acting elements that regulate expression of the spliced leader RNA gene in Leishmania amazonensis. Nucl. Acids Res. 22, 1959-1965
1992-1993
Agami, R. and Shapira, M. (1992). Sequence analysis of the spliced leader RNA from Leishmania mexicana amazonensis. Nucleic Acids Res., 20, 1804
Michaeli,S., Agami,R. and Shapira, M. (1993) Leishmania mexicana amazonensis, effect of heat shock on
Aly, R., Argaman,M., Agami, R., Pinelli, E. and Shapira, M. (1993) Intergenic sequences from the hsp83 gene cluster in Leishmania mexicana amazonensis promote and regulate gene expression in transfected parasites. Gene 127, 155-167
1988-1990
Shapira, M. and Pedraza, G. (1990) Heat shock protein 83 of Leishmania mexicana amazonensis, Sequence analysis and transcriptional activation. Mol. Biochem. Parasitol. 42, 247-256
Pinelli, E. and Shapira, M. (1990) Temperature induced expression of proteins in Leishmania mexicana amazonensis: A 22 kDa is possibly localized to the mitochondrion. Eur. J. Biochem. 194, 685-691
Shapira M., McEwen, J.G. and Jaffe, C.L. (1988) Temperature effects on molecular processes which lead to stage differentiation in Leishmania. EMBO 7, 2895-2901Shapira, M. and Pinelli, E. (1989) Heat shock protein 83 of Leishmania mexicana amazonensis is an abundant cytoplasmic protein with a tandemly repeated genomic arrangement. Eur. J. Biochem. 185, 231-236.
1982-1987
Shapira M., Homa F.L., Glorioso J.C., Levine M. (1987) Regulation of the herpes simplex virus type 1 late glycoprotein C gene: Sequences between base pairs -34 to +29 control transient expression and responsiveness to transactivation by the products of the immediate early (a) 4 and 0 genes. Nucleic Acids Research, 15, 3097-3111.
Shapira M., Misulovin Z., and Arnon R. (1985) Specificity and cross-reactivity of synthetic peptides derived from a major antigenic site of influenza hemagglutinin. Mol. Immunol. 22, 23-28
Shapira M., Jolivet M., and Arnon R. (1985) A synthetic vaccine against influenza with built-in adjuvanticity. J. Int. Immunopharmacol. 7, 719-723
Shapira M., Jibson M., Muller G., and Arnon R. (1984) Immunity and protection against influenza virus by synthetic peptide corresponding to antigenic sites of the hemagglutinin. Proc. Natl. Acad. Sci. USA. 81, 2461-2465
Arnon R., Shapira M., and Jacob C.O. (1983) Synthetic vaccines. J. Immunol.Methods 61, 261-273Muller G., Shapira M. and Arnon R. (1982) Anti-influenza response achieved by immunization with a synthetic conjugate. Proc. Natl. Acad. Sci. USA.79, 569-573.