Resources

Wet lab

The lab is fully equipped for protein purification, analysis, and storage. Our in-house instrumentation includes two FPLC systems, a glovebox dedicated to anaerobic work with iron-sulfur cluster-containing proteins, a multi-mode plate reader, incubators, centrifuges, electrophoresis equipment, spectrophotometers, -20 °C and -80 °C freezers, and liquid nitrogen dewars for the storage of cells and cryo-EM grids.

Through our central core facility, we also have access to advanced biophysical tools, including a fluorimeter for steady-state fluorescence measurements, a dynamic light scattering (DLS) system, isothermal titration calorimetry (nano-ITC), surface plasmon resonance (SPR), and microscale thermophoresis (MST) equipment.

Cryo-EM

Our lab plays a leading role in advancing cryo-electron microscopy in Israel through both active research and continuous infrastructure development. We are among the most frequent users of our institutional cryo-EM facility, where we routinely screen and analyze samples using a Thermo Scientific Glacios 200 kV microscope. This microscope is equipped with a Falcon 4i direct electron detector and will also feature a Selectris X energy filter.

Thanks to a national core grant secured under my leadership, a Titan Krios 300 kV microscope and a Hydra dual-beam FIB-SEM system are currently being installed. The Krios will be equipped with a Selectris X energy filter, enabling high-resolution single-particle analysis and in situ cellular cryo-electron tomography with enhanced image contrast and energy filtering capabilities.

Computational

The lab operates three GPU servers equipped with a total of 14 GPUs (ranging from 12 GB to 32 GB RAM) and approximately 760 TB of local storage for data processing and analysis.

We also have access—through our cryo-EM core facility—to two NVIDIA DGX A100 machines, each featuring 8× A100 GPUs (80 GB), 2 TB system RAM, and high-speed NVMe storage. These high-performance systems are optimized for protein structure prediction, high-resolution structure determination of large macromolecular complexes, and subtomogram averaging.

Code

Dedicated code developed in our lab to support our research is available at:

This repository contains tools and scripts related to our work on protein assembly and structural analysis.