The
IKI Cryo Electron Microscopy Unit provides expertise and resources for solving
macro-molecular structures. Structure determination is enabled by a high-end
transmission electron microscope (TEM), (FEI Glacios).
The
Thermo Fisher Scientific Glacios is a 200 kV transmission electron microscope
with X-FEG and Falcon 4i detector coupled to a Selectris X energy filter for
high-resolution structure determination of biological (and other soft matters)
macromolecules.
Its
12-grid autoloader allows efficient loading and unloading of cryo-EM grids. The
automated EPU and Tomo software systems are used for automated data collection,
capable of producing >300 movies/hour, for high-resolution single particle
analysis and cryo-electron tomography.
| Accelerating Voltage | 200 kV |
| Electron Source | X-FEG |
| Stage Tilt Range | ± 70° |
| C2 Apertures | 150, 100, 70 and 50 µm |
| Objective Apertures | 100, 70 and 30 µm |
| Operating Temperature | ~80 K |
| Information Limit at 0° / ±70° tilt | <0.2 nm / 0.34 nm |
| Cs | 2.7 mm |
| Number of cartridge positions for grids | 12 total |
| Sample exchange time | ~3 mins from autoloader cassette to stage |
| Camera | Falcon 4i |
| Camera pixels | 14×14 micrometers pixel size, 4096×4096 pixels, Electron Event Recording |
-
The Selectris X is designed for stability and atomic resolution imaging
-
Narrow (<10 eV) zero-loss energy filtering offers enhanced contrast for higher-resolution reconstructions
-
Aberration correction, low image distortions, and uniform energy resolution over the entire field of view