{"id":12,"date":"2018-02-24T10:58:48","date_gmt":"2018-02-24T10:58:48","guid":{"rendered":"https:\/\/lifewp.bgu.ac.il\/wp\/crystallography\/?page_id=12"},"modified":"2023-02-08T13:14:01","modified_gmt":"2023-02-08T11:14:01","slug":"facilities","status":"publish","type":"page","link":"https:\/\/lifewp.bgu.ac.il\/wp\/crystallography\/index.php\/facilities\/","title":{"rendered":"Facilities"},"content":{"rendered":"

The Macromolecular Crystallography Research Center has a state of the art facilities for setting up highthroughput crystallization experiments with a nano-liter volume of samples and for storing and monitoring the crystallization experiments at various temperatures.<\/p>\n

Mar<\/b>\u00b5X X-ray System (MarResearch)<\/b><\/div>\n
<\/div>\n
A complete home source for X-ray crystallography, which includes a state-of-the-art micro beam sealed tube generator with a specially designed high-performance optics, a mardtb “desktop beamline” and a \"mar345mar345= image plate detector, as well as cryogenic cooling system.  to it plus a cryo-cooler<\/div>\n
<\/div>\n
\"\"<\/div>\n
Specifications:<\/div>\n
Generator – 30 Watts (50 kV, 600 mA)<\/div>\n
Detector – mar345 image plate detector with 345 mm diameter<\/div>\n
Gonistat – mardtb “desktop beamline” with easymount<\/div>\n
Cryo cooler – Oxford Cryosystems<\/div>\n
\n\n\n\n\n\n\n\n\n\n
Beam size at sample:<\/td>\n\u2264 250 x 250 \u03bcm<\/td>\n<\/tr>\n
Beam divergence:<\/td>\n\u2264 5 x 5 mrad2<\/sup><\/td>\n<\/tr>\n
Total flux:<\/td>\n> 6 x 108<\/sup> photons\/sec<\/td>\n<\/tr>\n
Peak flux density at focal spot:<\/td>\n> 1 x 1010<\/sup> photons\/sec\/mm2<\/sup><\/td>\n<\/tr>\n
Focal distance:<\/td>\n600 mm from source<\/td>\n<\/tr>\n
Anode cooling:<\/td>\nair-cooling by fan<\/td>\n<\/tr>\n
Mirror protection:<\/td>\ndiaphragm vacuum pump with interlock to shutter<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n
<\/div>\n
Rock Maker (Formulatrix)<\/b>
\nPowerful software which provides the experimental design and which automates and tracks the crystallization process. It allows for the <\/span>rapid <\/span>creation of complex experiments and crystallization<\/span><\/div>\n
screens in a <\/span>user-friendly environment.<\/span><\/div>\n
\"\"<\/div>\n
Formulator (Formulatrix)<\/b>
\nA liquid handler that dispenses crystallization solutions for 24- as well as for 96-well grids rapidly and with high accuracy.<\/div>\n
 <\/span>\"\"  <\/span><\/div>\n
NT8 Drop Setter (Formulatrix)<\/b>
\nA fast and precise automated nanoliter dispenser for setting up crystallization experiments including sitting drops, hanging drops and seeding.<\/div>\n
\"\"<\/div>\n
Rock Imager (Formulatrix)<\/b>
\nAn automated imaging system for protein crystallization. This apparatus incubates and captures quality images of up to 250 crystallization plates on a user-defined schedule.<\/span><\/div>\n
\"\"<\/div>\n
\n

Rock Maker Web (Formulatrix)
\n<\/strong>Website for users to follow up on the crystallization experiments<\/p>\n<\/div>\n

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<\/div>\n

Computing Facilities
\n<\/strong>The MCRC has various workstations running under Linux operating system. Commonely crystallographic softwares for all the stages of a crystal structure determination (data collection, processing, phasing, refinement, molecular graphics, structure analysis and validation) and modelling are available for users to process and analyze their data.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

The Macromolecular Crystallography Research Center has a state of the art facilities for setting up highthroughput crystallization experiments with a nano-liter volume of samples and for storing and monitoring the crystallization experiments at various temperatures. Mar\u00b5X X-ray System (MarResearch) A complete home source for X-ray crystallography, which includes a state-of-the-art micro beam sealed tube generator with … <\/p>\n

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