Publications

2023

  • Michael Shaferman, Melis Gencel, Noga Alon, Khawla Alasad, Barak Rotblat, Adrian WR, Lital Alfonta, Shimon Bershtein, The fitness effects of codon composition of the horizontally transferred antibiotic resistance genes intensify at sub-lethal antibiotic levels. Mol. Biol. Evol. 2023, https://doi.org/10.1093/molbev/msad123
  • Hezi Hayun, Matt Coban, Ashok Kumar Bhagat, Eden Ozer, Lital Alfonta, Thomas R Caulfield, Evette S Radisky, Niv Papo, Utilizing genetic code expansion to modify N-TIMP2 specificity towards MMP-2, MMP-9, and MMP-14, Scientific Reports, 2023, https://doi.org/10.21203/rs.3.rs-2446107/v1
  • Anastasya Boyarski, Noam Shlush, Shiraz Paz, Jerry Eichler, Lital Alfonta, Electrochemical characterization of a dual-cytochrome containing lactate dehydrogenase, Bioelectrochemistry, 2023, 152, 108406. 

2022

  • Itay Algov, Aviv Feiertag, Rafi Shikler, Lital Alfonta, Sensitive enzymatic determination of neurotransmitters in artificial sweat, Biosens. Bioelectron. 2022, 210, 114264. 

2021

  • Itay Algov, Lital Alfonta, Use of protein engineering to elucidate electron transfer pathways between proteins and electrodes, ACS measurements Science Au, 2021, DOI:10.1021/acsmeasuresciau.1c00038. 
  •  Eden Ozer, Karin Yaniv, Einat  Chetrit, Anastasia Boyarski, Michael Meijler, Ronen Berkovich, Ariel Kushmaro, Lital Alfonta, An inside look at a biofilm: Pseudomonas aeruginosa flagella bio-tracking, Science Advances, 2021, 7, eabg8581.  
  • Itay Algov, Aviv Feiertag, Lital Alfonta, Site-specifically wired and oriented glucose dehydrogenase fused to a minimal cytochrome with high glucose sensing sensitivity, Biosens. Bioelectron. 2021, 180, 113117.
  • Eden Ozer, Lital Alfonta, Genetic Code Expansion of Vibrio Natriegens, Front. Bioeng. Biotechnol. 2021, 9, 594429. DOI: 10.3389/fbioe.2021.594429.
  • Lital Alfonta, Bioelectrochemistry and the Singularity Point: “I Robot”?”, Isr. J. Chem. Rosarium Philosophorum on Electrochemistry, 2021, 61, 1 – 9. DOI: 10.1002/ijch.202000113.

2020

  • Y. Chemla, M. Peeri, M. L. Heltberg, J. Eichler, M. H. Jensen, T. Tuller, L. Alfonta, A possible universal role for mRNA secondary structure in bacterial translation revealed using a synthetic operon, Nature Communications, 2020, 11, 4827, 1-11; https://doi.org/10.1038/s41467-020-18577-4.
  • M. Santhanam, I. Algov, L. Alfonta, DNA/RNA electrochemical biosensing devices a future replacement of PCR methods for a fast-epidemic containment, Sensors, 2020, 20, 4648.
  • Mor Fridman, Eden Ozer, Ariel Kushmaro, Lital Alfonta, Cellular localization of cytochrome bd in cyanobacteria using genetic code expansion, Biotechnol. Bioengineer. 2020, 117, 523-530.Selected as an Editor’s Choice
  • Ashok Kumar Bhagat, Hadar Buium, Guy Shmul, Lital Alfonta, Genetically Expanded Reactive Oxygen Tolerant Alcohol Dehydrogenase II, ACS Catalysis 2020, 10, 3094-3102. Features on the cover of issue 10.
  • Jiri Vacik, Vladimir Hnatowicz, Dietmar Fink, Geraldo Muñoz Hernandez, Humberto García Arellano, Arik Kiv, Lital Alfonta, Coupled chemical reactions in dynamic nanometric confinement: IX. Etched tracks with membranes made of calcium carbonate, Radiation Effects and Defects in Solids, 2020, 175, 7-25.

2019

  • Lin Xia, Ming-Jie Han, Lu Zhou, Aiping Huang, Zhaoya Yang, Tianyuan Wang, Fahui Li, Lu Yu, Changlin Tian, Zhongsheng Zang, Qing-Zheng Yang, Chenli Liu, Wenxu Hong, Yi Lu, Lital Alfonta, and Jiangyun Wang, S-Click Reaction for Isotropic Orientation of Oxidases on Electrodes to Promote Electron Transfer at Low Potentials. Angew. Chem. Int. Ed. 2019, 58, 1-6. DOI: 10.1002/anie.201909203, Selected as a Hot paper.
  • Yonatan Chemla, Eden Ozer, Michael Shaferman, Ben Zaad, Rambabu Dandela, Lital Alfonta, Simplified Methodology for a Modular and Genetically Expanded Protein Synthesis in Cell-Free Systems, Synthetic and Systems Biotechnology, 2019, 4, 189-196.

2018

  • Orr Schlesinger, Rambabu Dandela, Ashok Bhagat, Raju Adepu, Michael M. Meijler, Lin Xia, Lital Alfonta, Phothoswitchable microbial fuel cells, Biotechnol. Bioengineer. 2018,115 (5),1355-1360, DOI: 10.1002/bit.26555.
  • Humberto Garcia Arellano, Gerardo Munoz Hernandez, Dietmar Fink, Jiri Vacik, Vladimir Hnatowicz, Lital Alfonta, Arik Kiv, Dependence of Yield of Nuclear Track-Biosensors on Track Radius and Analyte Concentration, Nuclear Inst. and Methods in Physics Research, B, 2018,420,69-75.
  • Orr Schlesinger, Mor Pasi, Rambabu Dandela, Michael M. Meijler, Lital Alfonta, Electron Transfer Rate Analysis of a Site-Specifically Wired Copper Oxidase, Physical Chemistry Chemical Physics, 2018,20, 6159-6166. DOI: 10.1039/C8CP00041G.
  • Dietmar Fink, Gerardo Munoz Hernandez, Humberto Garcia Arellano, Jiri Vacik, Vladimir Hnatowicz, Arik Kiv, Lital Alfonta, Ion track etching revisited: I. Correlations between track parameters in aged polymers, Nuclear Inst. and Methods in Physics Research, B, 2018,420,57-68.
  • Humberto Garcia Arellano, Gerardo Munoz Hernandez, Dietmar Fink, Jiri Vacik, Vladimir Hnatowicz, Arik Kiv, Lital Alfonta, Ion Track Etching Revisited: II. Electronic Properties of Aged tracks in Polymers, Rad. Effects Def. Solids, 2018,173 ,148-164. DOI: 10.1080/10420150.2018.1442454.
  • Yonatan Chemla, Eden Ozer, Itay Algov, Lital Alfonta, Context effects of genetic code expansion by stop codon suppression, Current Opinion in Chemical Biology. 2018,46, 146-155.
  • Orr Schlesinger, Lital Alfonta, Encapsulation of Microorganisms, Enzymes, and Redox Mediators in Graphene Oxide and Reduced Graphene Oxide, Methods in Enzymology. 2018, https://doi.org/10.1016/bs.mie.2018.05.008.

2017

  • Eden Ozer, Yonatan Chemla, Orr Schlesinger, Haim Yuval Aviram, Inbal Riven, Gilad Haran, Lital Alfonta, In-vitro suppression of two different stop codons, Biotechnol. Bioengineer. 2017, 114 , 1065-1073. Highlighted as an Editor’s Choice and features as a cover page of issue 114(5)
  • Orr Schlesinger, Yonatan Chemla, Mathias Heltberg, Eden Ozer, Ryan Marshall, Vincent Noireaux, Mogens Høgh Jensen, Lital Alfonta, Tuning of recombinant protein expression in Escherichia coli by manipulating transcription, translation initiation rates and incorporation of non-canonical amino acids, ACS Synth. Biol. 2017, DOI: 10.1021/acssynbio.7b00019
  • Dietmar Fink, Jiri Vacik, Vladimir Hnatowicz, G. Muñoz Hernandez, H. Garcia Arrelano, Lital Alfonta, Arik Kiv, Diffusion kinetics of the glucose/glucose oxidase system in swift heavy ion track-based biosensors, Nuclear Instr. Meth. Phys. Res. B. 2017, 398 , 21-26.
  • Yonatan Chemla, Mor Friedman, Mathias Heltberg, Anna Bakhrat, Elad Nagar, Rakefet Schwarz, Mogens Høgh Jensen, Lital Alfonta, Expanding the genetic code of a photo-autotrophic organism, Biochemistry, 2017, 56, 2161-2165
  • Alon Szczupak, Dror Aizik, Sarah Moraïs, Yael Vazana, Yoav Barak, Ed Bayer, Lital Alfonta, The Electrosome: A Surface-Displayed Enzymatic Cascade in a Biofuel Cell’s anode and a High-Density Surface-Displayed Biocathodic Enzyme,Nanomaterials, 2017,doi:10.3390/nano7070153
  • Itay Algov, Jennifer Grushka, Raz Zarivach, Lital Alfonta, Highly Efficient Flavin–Adenine Dinucleotide Glucose Dehydrogenase Fused to a Minimal Cytochrome C Domain, J. Am. Chem. Soc, 2017,139 (48)56,17217–17220, DOI: 10.1021/jacs.7b07011

2016

  • Nataliia Guz, Tatiana A. Fedotova, Brian E. Fratto, Orr Schlesinger, Lital Alfonta, Dmitry M. Kolpashchikov, Evgeny Katz, Bioelectronic Interface Connecting Reversible Logic Gates Based on Enzyme and DNA Reactions, ChemPhysChem , 2016, DOI: 10.1002/cphc.201600129.
  • Dietmar Fink, Gerardo Munoz Hernandez, Humberto Garcia Arellano, Jiri Vacik, Vladimir Havranek, Vladimir Hnatowicz, Arik Kiv, Lital Alfonta, Nuclear Track-Based Biosensing: an Overview, Rad. Effects Def. Solids 2016, 171, 173-185.
  • Idan Gal, Orr Schlesinger, Liron Amir, Lital Alfonta, Yeast Surface Display of Dehydorgenases in Microbial Fuel Cells, Bioelectrochem. 2016, 112, 53-60. One of the most downloaded papers of Bioelectrochemistry on 2016.
  • Dan Bar-Yaacov, Idan Frumkin, Yuka Yashiro, Takeshi Chujo, Yuma Ishigami, Yonatan Chemla, Amit Blumberg, Orr Schlesinger, Philipp Bieri, Basil Greber, Nenad Ban, Raz Zarivach, Lital Alfonta, Yitzhak Pilpel, Tsutomu Suzuki, Dan Mishmar, Mitochondrial 16S rRNA Is Methylated by tRNA Methyltransferase TRMT61B in All Vertebrates, PloSBiol, 2016, DOI:10.1371/journal.pbio.1002557.
  • Shunin, Yu., Fink, D., Kiv, A., Alfonta, L., Mansharipova, A., Muhamediyev, R., Zhukovskii, Yu., Lobanova-Shunina, T., Burlutskaya, N., Gopeyenko, V. Bellucci, S. Theory and modelling of real-time physical and bio-nanosensor systems,Co,puter Modelling and New Technologies, 2016,20 ,7–17.

2015

  • Lin Xia, Yehonatan Ravenna, Lital Alfonta, Layer-by-layer assembly of a redox enzyme displayed on the surface of an elongated bacteria into a hierarchical artificial biofilm based anode, Chem. Commun. 2015, 51, 2633 – 2636.
  • Yonatan Chemla, Eden Ozer, Orr Schlesinger, Vincent Noireaux, Lital Alfonta, Genetically expanded cell-free protein synthesis using endogenous pyrrolysyl orthogonal translation system, Biotechnol. Bioengineer. 2015, 112 , 1663-1672.
  • Yehonatan Ravenna, Lin Xia, Jenny Gun, Alexey Mikhaylov, Alexander Medvedev, Ovadia Lev, Lital Alfonta, Biocomposite Based on Reduced Graphene Oxide Film Modified with Phenothiazone and Flavin Adenine Dinucleotide-Dependent Glucose Dehydrogenase for Glucose Sensing and Biofuel Cell Applications, Anal. Chem. 2015, 87, 9567-9571.
  • 31. D. Fink, J. Vacik, V. Hnatowicz, G. Muñoz H., H. García Arellano, A. Kiv, L. Alfonta, Coupled chemical reactions in dynamic nanometric confinement: IV. Ion transmission spectrometric analysis of nanofluidic behavior and membrane formation during track etching in polymers,Red.Effects Def. Solids. 2015, 170 , 155-174-1672.

2014

  • D. Fink, J. Vacik, H. G. Arellano, G. H.  Muñoz, L. Alfonta, W. R. Fahrner, K. Hoppe, A. Kiv, Biosensors with Nuclear Tracks and Embedded Membranes, Key Engineering Materials 2014, 605, 83-86.
  • H. Garcıa-Arellano, Dietmar Fink, Geraldo Munoz Hernandez, Jiri Vacık, Vladimir Hnatowicz, Lital Alfonta, Nuclear track-based biosensors with the enzyme laccase, App. Surf. Sci. 2014, 310, 66-76.
  • Lital Alfonta, Brian Meckes, Liron Amir, Orr Schlesinger, Srinivasan Ramachandran, Ratnesh Lal, Measuring Localized Redox Enzyme Electron Transfer in a Live Cell with Conducting Atomic Force Microscopy, Anal. Chem. 2014, 86, 7674-7680.

2013

  • Yohai Mandabi, Dietmar Fink, Lital Alfonta, Label-free DNA Detection Using the Narrow Side of Funnel-type Etched Nanopores, Biosens. Bioelectron. 2013, 42, 362-366.
  • Liron Amir, Stewart A. Carnally, Josep Rayo, Shaked Rosenne, Sarit Melamed Yerushalmi, Orr Schlesinger, Michael M. Meijler,Lital Alfonta, Surface Display of a Redox Enzyme and its Site Specific Wiring to Gold Electrodes, J. Am. Chem. Soc. 2013, 135, 70-73, recommended as being of special significance in its field by James Canary and Xiaojian Wang from the F1000 Faculty.
  • Liang Xiang, Kathryn Moncivais, Faqin Jiang, Lital Alfonta, Zhiwen J. Zhang, Crucial Optimization of Translational Components towards Efficient Incorporation of Unnatural Amino Acids into Proteins in Mammalian Cells, PLoSONE. 2013, 8(7) : e67333. doi:10.1371/journal.pone.0067333.
  • Sviatlana Smolskaya, Zhiwen J. Zhang, Lital Alfonta, Enhanced Yield of Recombinant Proteins with Site-specifically Incorporated Unnatural Amino Acids Using a Cell-Free Expression System, PLoSONE. , 2013, 8(7): e68363. doi:10.1371/journal.pone.0068363
  • G. Muñoz Hernandez, S. A. Cruz, R. Quintero, H. García Arellano, D. Fink, L. Alfonta, Y. Mandabi, A. Kiv, J. Vacik, Coupled chemical reactions in dynamic nanometric confinement: Ag2O membrane formation during ion track etching,Red. Effects Def. Solids . 2013, 168, 675-695.

2012

  • Dietmar Fink, Gerardo Munoz Hernandez, Lital Alfonta, Highly sensitive urea sensing with ion-irradiated polymer foils, Nuclear Instr. Meth. Phys. Res. B 2012, 273, 164-170.
  • D. Fink, J. Vacik, L. Alfonta, A. Kiv, Y. Mandabi, Optimization of transport processes in etched track-based biosensors, Rad. Effects Def. Solids,2012, DOI:10.1080/10420150.2011.647914.
  • Alon Szczupak, Dan Kol-Kalman, Lital Alfonta,A hybrid biocathode: surface display of O2-reducing enzymes for microbial fuel cell applications, Chem. Commun. 2012, 48, 49-51, Selected as a hot paper by the editor and as a back cover p.163.
  • Lenka Halámková, Jan Halámek, Vera Bocharova, Alon Szczupak, Lital Alfonta, Evgeny Katz, Implanted biofuel cell operating in living snail, J. Am. Chem. Soc. 2012, 134, 5040-5043, highlighted in Nature:doi:10.1038/nature.2012.10210.
  • Alon Szczupak, Jan Halámek, Lenka Halámková, Vera Bocharova, Lital Alfonta, Evgeny Katz, Living battery – biofuel cells operating In Vivo in clams, Energy & Environ. Sci. 2012, 5, 8891-8895, Features on the Cover of issue 10.
  • Karnit Bahartan, Liron Amir, Alvaro Israel, Rachel G. Lichtenstein, Lital Alfonta, In situ fuel processing in a microbial fuel cell, ChemSusChem 2012, 9, 1820-1825, inside cover of ChemSusChem p. 1642.
  • Karnit Bahartan, Jenny Gun, Sergey Sladkevich, Petr V. Prikhodchenko, Ovadia Lev, Lital Alfonta, Encapsulation of Yeast Displaying Glucose Oxidase on their Surface in a Graphene Oxide Hydrogel Scaffolding and its Bioactivation, Chem. Commun. 2012, 48, 11957-11959.

2011

  • D. Fink, G. Muñoz H., J. Vacik,L. Alfonta 2011,  Pulsed Biosensing, IEEE sensors Journal 11(4), 1084-1087.
  • Dietmar Fink, Gerardo M. Hernandez, Lital Alfonta,2011, Ion track based urea sensing, Sensor Actuat. B-Chem. 156, 467-470,

2010

  • Lital Alfonta, 2010, Genetically Engineered Microbial Fuel Cells, Electroanalysis, 22, 822-831.
  • Gabrielle Nina Thibodeaux, Xiang Liang, Kathryn Moncivais, Aiko Umeda, Oded Singer, Lital Alfonta, Zhiwen Jonathan Zhang*. 2010, Transforming an orthogonal tRNA-aminoacyl-tRNA synthetase pairs from Archaea previously evolved in E. coli to function in mammalian cells, PLoSONE, 5(6): e11263. doi:10.1371/journal.pone.0011263.
  • Dietmar Fink, Jiri Vacik, Vladimir Hnatowicz, Gerardo Muñoz,L. Alfonta, I. Klinkovich, 2010, Funnel-type etched ion tracks in polymers, Rad. Effects Def. Solids, 165, 343-361.
  • Gerardo Muñoz Hernandez Irina Klinkovich, Lital Alfonta, Dietmar Fink, 2010, Glucose sensing with complex polymer-electrolyte systems, IEEE sensors Journal, 10(12), 1849-1854.

2009

  • Dietmar Fink, Irina Klinkovich, Ohad Bukelman, Robert S. Marks, Arik Kiv, David Fuks, Wolfgang Fahrner, Lital Alfonta 2009, Glucose determination using a re-usable ion track membrane sensor, Biosens. Bioelectron.24, 2702-2706.
  • Lital Alfonta, Ohad Bukelman, Amita Chandra, Wolfgang R. Fahrner, Dietmar Fink, David Fuks, Vladimir Golovanov, Vladimir Hnatowicz, K. Hoppe, Arik Kiv, Irina Klinkovich, Meron Landau, Joan Ramon Morante, Nikolai V. Tkachenko, Jiri Vacík, Mika Valden, 2009, Strategies towards Advanced Ion Track-Based Biosensors, Rad. Eff. Def. Solids, 164, 431-437.
  • Liron Amir, Tsz Kin Tam, Marcos Pita, Michael M. Meijler, Lital Alfonta, Evgeny Katz*, 2009, Biofuel Cell Controlled by Enzyme Logic Systems, J. Am. Chem. Soc, 131, 826-832.
  • Ohad Bukelman, Neri Amara, Roi Mashiach, Pnina Krief, Michael Meijler*, Lital Alfonta, 2009, Electrochemical Analysis of Quorum Sensing Inhibition, Chem. Commun. 20, 2836-2838. Back Cover p. 2951
  • Simon Fishilevich, Liron Amir, Yearit Fridman, Amir Aharoni, Lital Alfonta, 2009, Surface Display of Redox Enzymes in Microbial Fuel Cells, J. Am. Chem. Soc.131, 12052-12053. Pubget’s hot paper of May 2012.

2007

  • Wenshe Liu, Lital Alfonta, Antha V. Mack and Peter G. Schultz; 2007, Structural Basis for the Recognition of para-Benzoyl-L-phenylalanine by Evolved Aminoacyl-tRNA Synthetases. Angew. Chem. Intl. Ed. 46, 6073-6075.

2004

  • Lital Alfonta, Immanuel Blumenzweig, Maya Zayats, Lea Baraz, Moshe Kotler and Itamar Willner; 2004, Electronic Transduction of HIV-1 Drug Resistance in AIDS Patients.ChemBioChem.5, 949-957.
  • Zhiwen Zhang, Lital Alfonta, Feng Tian, Badry Bursulaya, Sean Uryu, David. S. King and Peter G. Schultz; 2004, Selective Incorporation of 5-hydroxytryptophan into Proteins in Mammalian Cells. Proc. Natl. Acad. Sci. USA101, 8882-8887.

2003

  • Lital Alfonta, Zhiwen Zhang , Sean Uryu, Joseph A. Loo and Peter G. Schultz; 2003, Site-Specific Incorporation of a Redox Active Amino Acid into Proteins. J. Am. Chem. Soc.125, 14662-14663.

2001

  • Lital Alfonta, Anup K. Singh and Itamar Willner; 2001, Liposomes Labeled with Biotin and Horseradish Peroxidase: A New Probe for the Enhanced Amplification of Antigen-Antibody or Oligonucleotide-DNA Sensing Processes by the Precipitation of an Insoluble Product on Electrodes. Anal. Chem.73, 91-102.
  • Eugenii Katz, Lital Alfonta and Itamar Willner; 2001, Chronopotentiometry and Faradaic Impedance Spectroscopy as Methods of Signal Transduction in Immunosensors. Sensor Actuat. B-Chem.76, 134-141.
  • Andrei B Kharitonov, Maya Zayats, Lital Alfonta, Eugenii Katz and Itamar Willner; 2001, A novel ISFET-based NAD+ – dependent Enzyme Sensor for Lactate. Sensor Actuat. B-Chem.76, 203-210.
  • Lital Alfonta, Amos Bardea, Olga Khersonsky, Eugenii Katz and Itamar Willner; 2001, Chronopotentiometry and Faradaic Impedance Spectroscopy as Signal Transduction Methods for the Biocatalytic Precipitation of an Insoluble Product on Electrode Supports: Routes for Enzyme Sensors, Immunosensors and DNA-Sensors. Biosens. Bioelectron.16, 675-687. (Editor’s Selection).
  • Lital Alfonta, Itamar Willner, Dan J. Throckmorton and Anup K. Singh; 2001, Electrochemical and Quartz Crystal Microbalance Detection of the Cholera Toxin Employing Horseradish Peroxidase And GM1-Functionalized Liposomes, Anal. Chem. 73, 5287-5295.
  • Lital Alfonta and Itamar Willner; 2001 Electronic Transduction of Biocatalytic Transformations on Nucleic Acid Functionalized Surfaces, Chem. Commun.16, 1492-1493.

2000

  • Lital Alfonta, Eugenii Katz and Itamar Willner; 2000, Sensing of Acetylcholine by a Tri-Component-Enzyme-Layered Electrode Using Faradaic Impedance Spectroscopy, Cyclic Voltammetry and Microgravimetric Quartz-Crystal-Microbalance Transduction Methods, Anal. Chem.5, 927-935.
  • Andrei B. Kharitonov, Lital Alfonta, Eugenii Katz and Itamar Willner; 2000, Probing of Bioaffinity Interactions at Interfaces Using Impedance Spectroscopy and Chronopotentiometry. J. Electroanal. Chem.487, 133-141.